Biological and physico-mechanical properties of poly(methyl methacrylate) enriched with graphene oxide as a potential biomaterial.

Objective: To determine the cytotoxicity and effects of graphene oxide (GO) on cellular proliferation gingival-fibroblasts, pulpdental cells human osteoblasts in culture, to physical, mechanical biological properties poly (methyl methacrylate) (PMMA) enriched with GO. Material Methods: T he G O w as c haracterized SEM. Cytotoxicity cell were determined by MTT bioassay. The physical-mechanical tests (flexural strength elastic modulus) carried out a universal testing machine. Sorption solubility weighing before after drying immersion water. Porosity was evaluated visual inspection. Data analyzed Student's t-test Tukey's post-hoc ANOVA. Results: GO has heterogeneous morphology particle size 66.67±64.76 μm. slight no-cytotoxicity (>50-75% viability) at 1-30 days, 24 hours incubation PMMA significantly stimulates (45±8%, p<0.01). physical increase considerably without altering sorption, porosity. Conclusion: alone or an acceptable biocompatibility, could contribute proliferation, regeneration improve PMMA.